Kikuchi's other notable films include Norwegian Wood (2010), which screened in competition at the 67th Venice Film Festival and Guillermo del Toro's science fiction action film Pacific Rim (2013). She was the first Japanese actress to be nominated for an Academy Award in 50 years, for her work in Babel (2006). Rinko Kikuchi ( 菊地 凛子, Kikuchi Rinko, born Yuriko Kikuchi ( 菊地 百合子, Kikuchi Yuriko), January 6, 1981) is a Japanese actress. For more guidance, see Wikipedia:Translation.You should also add the template to the talk page. A model attribution edit summary is Content in this edit is translated from the existing Japanese Wikipedia article at ] see its history for attribution. You must provide copyright attribution in the edit summary accompanying your translation by providing an interlanguage link to the source of your translation.If possible, verify the text with references provided in the foreign-language article. Do not translate text that appears unreliable or low-quality.Consider adding a topic to this template: there are already 3,695 articles in the main category, and specifying |topic= will aid in categorization.Machine translation, like DeepL or Google Translate, is a useful starting point for translations, but translators must revise errors as necessary and confirm that the translation is accurate, rather than simply copy-pasting machine-translated text into the English Wikipedia.View a machine-translated version of the Japanese article.
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A confirmation email with Zoom login information will be sent closer to the open house date. in the Media Innovation Center at Evansdale Crossing. Most sessions will be recorded for research purposes only. The Game Design and Interactive Media program at WVU is hosting a Demo Day on Decemfrom 11 a.m. Note: Many of the tutors for the online Math Learning Center are funded as part of a research grant. Go to your app store and install the Microsoft Teams application to use Teams on your smartphone. The online Math Learning Center is available Sunday-Thursday from 7pm-10pm on Zoom. These are built into many laptops, but you can also connect external speakers or headset to your computer. The webinar will go live approximately 10 minutes before the conversation starts. To join an online meeting, attendees will need access to speakers and a microphone. Staff, faculty and students are invited to attend a Campus Conversation Tuesday (June 27) designed to provide an update on the University’s overarching transformation initiatives, including those to address academics and the structural budget deficit. Though it is not available to most students, Teams lets you schedule or join online meetings for up to 250 WVU colleagues using the web or desktop and mobile applications. Non-teaching WVU employees are encouraged to use Microsoft Teams if storing, sharing and collaborating on documents with colleagues is a priority. ITS is working to integrate Zoom with eCampus. Students with poor internet service can use phones to connect to meetings. User ID: PIN/Password: Skip to top of page. To claim your account or change your password, go to. Breakout groups can be created for collaboration. Enter your WVU username and password to log in. Zoom lets you connect live, via audio and video, and share presentation content. Just log in with your WVU credentials and authenticate through single sign-on, as you do with other services. The virtual information sessions will alternate between 12. Each information session will last one hour, include a brief overview of the College of Law and admissions process, and provide time for a general question-and-answer period. Resources on how to use Zoom are available online. Staff, faculty and students are invited to attend a Campus Conversation Tuesday (June 27) designed to provide an update on the University’s overarching transformation initiatives, including those to address academics and the structural budget deficit. The WVU Law Enrollment Management Office will be hosting information sessions via Zoom for prospective law students on Wednesdays during the Spring 2022 semester. Agendas and login information can be found at /agendas. 14 and 15) at the Erickson Alumni Center. Zoom, a real-time option for video conferencing, online meetings and collaboration, is now available to all WVU faculty, staff and students. The West Virginia University Board of Governors will meet Thursday and Friday (Sept. It ran for five seasons from 2015 to 2020. A French, contemporary Mad Men with counterrorism instead of advertising. If anything, the show’s polished dialogue, Guillaume’s unstable identity, and Guillaume and Marina’s mentor-mentee relationship will remind you of Mad Men. The offices and wardrobes are nondescript in a way that feels meticulously planned, and the show’s workplace drama style puts the characters’ ethical dilemmas front and center. Of course The Bureau is heightened for TV, but it feels so real. Further seasons get even deeper into the personal and professional lives of Guillaume, Marina, and other DGSE staffers. Meanwhile, he’s trying to locate an undercover agent named Cyclone, who has gone missing in Algeria, and training a young agent named Marina (Sara Giraudeau) for her upcoming undercover mission in Iran. He’s having a hard time readjusting to his real life, which gets even more complicated when Nadia (Zineb Triki), his Syrian girlfriend who only knows him as his alias, joins him in Paris. This French series about the inner workings of the DGSE - France’s version of the CIA - is TV’s most emotionally and narratively grounded spy series this side of The Americans.Īmélie’s Mathieu Kassovitz stars as intelligence officer Guillaume Debailly, who returns home to Paris after six years undercover in Syria. If you’re looking for a realistic spy show, The Bureau is practically a documentary. This list will self-destruct after you read it, so get streaming right away. Instead, I’m highlighting international series and domestic hits you may have missed. You don’t need a recommendation to watch classics like The Americans or Alias - you know all about them already. This highly classified list features the 11 best spy TV shows you may not have seen before. Because there’s nothing like a globetrotting, high-stakes thriller about people who work for an alphabet agency. You want to watch secret agents carry out thrilling missions using all the tools at their disposal, from computer hacking to hand-to-hand combat. Thinking they’ve hit the big time, it’s not long before word travels across the galaxy, and soon other crews go on the hunt for the space sweepers in hope of stealing the robot for themselves.Polygon is diving into the world of espionage throughout fiction and pop culture history with Deep Cover, a two-week special issue covering all sorts of spy stories and gadgets.Īmid all this spyjinx, you might find yourself wanting to go deeper into the TV spy genre. A team of space sweepers close to collapse suddenly find their luck changed when they find a human-like robot. In the not-so-distant future, Earth is on the brink of destruction, and the only form of currency left is the trash that remains. Millions of subscribers tuned in to watch Space Sweepers, which made the film a smash hit on the platform, and had many of the top ten lists around the world feature the space blockbuster.Īn incredibly fun, and extremely well-made movie, it’s astonishing what director Jo Sung-hee and his team at Bidangil Pictures could produce on a humble budget of only $21.2 million. Regarded as Korea’s first space blockbuster, it was important to have as many eyes on the movie as possible, which Netflix provided in droves. The release of Space Sweepers was not only a massive deal for Netflix, but also for South Korean cinema. However, the chance of becoming a millionaire also comes at the risk of losing your life and your humanity. Hundreds of contestants, all in deep financial hardship, are invited to take part in a selection of survival games which will see the winner earn themselves millions of dollars. If you haven’t already watched it, then please don’t miss out on watching the biggest Netflix show of the year. Some of the games seen in the show, such as Red Light Green Light have been emulated, and can now be found on such sites as Roblox and video games such as Overwatch. You can’t go five minutes without browsing on YouTube or TikTok before you’ll see some reference or video of Squid Game. This means that Squid Game will be more popular than Stranger Things, Tiger King, The Witcher, Lupin, Money Heist, and Bridgerton. However, we certainly weren’t expecting just how popular Squid Game was truly going to be.Īt the time of writing Squid Game has only been available to stream on Netflix for just over three weeks, but the series is on track to become the most-watched Netflix Original in Netflix’s eight-year history of original content. In our preview for Squid Game, we predicted that it would be the biggest K-Drama to land on Netflix since the release of the zombie-horror series Kingdom. With 2021’s slate being so big, we thought we’d pick out some of our favorite new K-drama additions this year. It’s thanks to Netflix’s investment of hundreds of millions of dollars into South Korean content which had made some of the titles in the list available to stream. The Free plan allows collaboration of up to 3 users.A 14-day free trial of the Pro plan is also available if you want to try out the software before committing to a plan. Customers can opt to pay a premium plan yearly and save 28% or pay month by month. All plans include unlimited tasks and projects. TrackingTime pricing is available in several plans including a free plan. Other reports include client reports and payroll reports. User reports show team member performance, while project reports display progress and budgets. TrackingTime also includes several types of reports. Employees can send time-off requests and get notified of its approval, and admins can create a list of leave types for teams to choose when requesting time-offs. The time tracking software can also work as an attendance tracking system. Additionally, users can export their time cards in PDF, CSV, and XLS formats. The software also sends emails to notify admins of pending approvals. It sends email reminders for users to complete their entries, and employees can easily submit their time cards after they verify it for accuracy. TrackingTime automatically generates employee time cards based on time entries. Time Cards, Attendance Tracking, Reporting, and More Users can track time and sync with tools they work on without having to switch apps. For instance, it integrates with Airtable, Asana, Basecamp, ClickUp, Evernote, GitHub, Google Workspace, and Jira, among others. Teams can collaborate by attaching files per project, organizing them, and sharing them from a central hub, and they can create custom project labels for additional reporting information.įor teams that are using other project management and productivity tools, TrackingTime integrates with over 50 apps for seamless time tracking. It has several views that include list, board, project, and timeline views to help a project manager see the bigger picture and prioritize needed tasks. TrackingTime includes simple project management functionality to give users an overview of how tasks are progressing. Timesheets are also customizable to include relevant information for reporting - and to make things even easier, users can save these as templates. The timesheets contain accurate data to help companies make informed business decisions, and automatic time audits ensure timesheet data is consistent and complete. Its online timesheets enable companies to follow internal processes minus the paperwork and manual efforts. Plus, it can track time by blocks according to workflows with automatic identification and reporting. It can track automatically, but it can also accommodate manual entries and edits. It’s compatible with web, desktop, and mobile platforms. TrackingTime provides flexible time tracking for team members to record their work time in a way that matches their work style, location, or work arrangement - by hour or by project. TrackingTime Features and Strengths Time Tracking and Online Timesheets
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Star activity (or off-target cleavage) and incomplete cleavage are potential challenges which may occur due to suboptimal enzymatic conditions or inappropriate enzyme storage. For complete digestion, make sure that the enzyme volume is 1/10th of the total reaction volume, the optimal temperature is constantly maintained throughout the reaction, the total reaction time is appropriately calculated based on the amount of DNA to be digested, appropriate buffers should be used to ensure maximal enzymatic activity, and in case of a double digest, make sure that the two restriction sites are far enough so that the activity of one enzyme cannot interfere with the activity of the other. Always use a control DNA digestion with the enzyme to ensure adequate activity (to avoid interference due to high glycerol in the enzyme). The enzyme should always be stored at -20C and multiple freeze-thaw cycles should be avoided in order to maintain optimal activity. The most common challenges with restriction digest include- 1. The four most common types of restriction enzymes include: Type I (cleaves at sites remote from a recognition site), Type II (cleaves within or at short specific distances from a recognition site), Type III (cleave at sites a short distance from a recognition site), and Type IV (targets modified DNA- methylated, hydroxymethylated and glucosyl-hydroxymethylated DNA). GSM7091149_Balbc_da圓_macrophage_rep2_rpkm.Restriction Enzymes NotI A restriction enzyme or restriction endonuclease is defined as a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at or near that site, known as restriction site or target sequence. Skeletal muscle regeneration failure in ischemic-damaged limbs is associated with pro-inflammatory macrophages and premature differentiation of satellite cells Pro-inflammatory macrophages impair skeletal muscle regeneration in ischemic-damaged limbs by inducing precocious differentiation of satellite cells Supplementary files format and content: indexed binary format files include RPKM values for each sample The bigwig files were generated using bamCoverage from alignment of reads (parameter-normalizeUsing:RPKM ). The reads from fastq files were aligned to GRCm38 reference genome using STAR v2.7.4a (parameter-sjdbOverhang: 99) DNA fragments between 400-600bp were selected by gel extraction using Zymoclean Gel DNA recovery kit (Zymo, #D4002). Library PCR was performed using unique combinations of Nextera-PCR i5/i7 primers. A Zymo DNA clean and concentrator kit (Zymo, R1014 ) was utilized (PMID: 24385147). DNA was digested by NotI-HF (NEB, #R3189L), tagmented using Tn5 assembled with adaptors Tn5ME-A/Tn5MErev and Tn5ME-B/Tn5MErev. PCR preamplification of cDNA was performed with IS PCR and Tn5ME-A-aHic using 2X KAPA PCR mix (Kapa Biosystem, KK2602) followed by cleanup using SPRISelect beads (Beckman Coulter, REF B23319). Total RNA was extracted using TRIzol Reagent (Invitrogen) according to manufacturer’s protocol.įirst-strand reverse transcription and template switching was performed using an Oligo(dT) primer (dT30VN-ME-A), a locked nucleic acid-containing TSO (NotI-TSO), and Superscript IV reverse transcriptase (Invitrogen, # 18090050). Approximately 100,000 macrophages for collected from one mouse for each biological replicate. Macrophages were isolated by fluorescence-activated cell sorting (SonySorter SH800s) with gating for PI-/CD45+/CD11b+/F480+ cells. Streptavidin-PE/Cy7 (Biolegend, 405206) was used as a secondary reagent for anti-F4/80-biotin (PMID: 25896247). Primary antibody staining was performed using anti-CD45-Alexa Fluor 488 (clone HI30, Invitrogen, MHCD4520), anti-CD11b (clone M1/70, Invitrogen, 12-0112-81), and anti-F4/80-biotin (clone A3-1, Bio-rad, MCA497BT) antibodies for 40 minutes. Cells were blocked with purified anti-mouse CD16/32 antibody (Biolegend, #101301) for 10 minutes. Single-cell suspensions were generated from skeletal muscle tissue using mechanical dissociation and enzymatic digestion with 0.05% Pronase (Sigma, 537088) for one hour. Macrophages from ischemic hindlimb skeletal muscle were isolated on post-op day 3 for RNA-seq analysis. Hindlimb ischemia surgery was performed on young adult male mice as previously described (PMID: 18285563). GEO help: Mouse over screen elements for information. Riberac has, reputedly, the department's biggest market (on a Friday) and Ste Foy La Grande's Saturday market is well worth a visit.įrom Bergerac along the Dordogne valley through Lalinde, to Sarlat there is an almost endless parade of breathtaking towns and villages.įoodies will find plenty to please them including the locally produced ducks and foie gras, walnuts and prunes and wines from the world-famous Bergerac vines.įorget any stereo-typing you've heard. Finding the perfect property in France can be a rewarding experience but. The house does not include the gaarge and consists of a narrow pointed stone tower like. Don't miss the excellent Vèsunna Gallo-Roman museum. Magical 18th Century Chateau For Sale with Maxwell. An old 3 bedroom stone house with a small garden high in a small historic market town in the Prigord vert. 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Whereas people may have traditionally purchased holiday homes, overseas retirement havens or sound investments in France, it is not so unusual to find the once career hungry individuals who have had enough of the city lifestyle opting for ‘the good life’ and buying a charming rustic chalet in a bustling French ski resort or running a successful business on the south coast of France. All the price on our website is calculated with an exchange rate of 1 pound 1.1600846249251513 Euros. 6 Bedrooms 6 Bathrooms Habitable Size: 563 m² Land Size: 67.4 ha. Full of character and charm and beautifully presented, this spacious 6 bedroom chateau, with a spacious total built area of 563m2, is ideally situated in a quiet setting in. Best properties for sale in Dordogne, Lot et Garonne and Lot. Location: Aquitaine, Dordogne (24), Montignac. Now people are buying all over the French continent depending on their individual requirements. Property In Dordogne Perigord France - Every week we put new properties in Dordogne. Historically, areas such as the Cote d'Azur and the Dordogne were popular with those looking to step onto the French property ladder. As property in France has become more accessible and affordable, many people have looked to invest. For some, the chance to own a second home or holiday villa in France is no longer a dream or a right that’s reserved for the rich and famous. Low-cost airlines that fly to smaller, local French airports combined with rising UK property prices are contributing factors for the surge in interest in the French property market. As such, France has experienced a surge in the number of Britons taking the plunge across the channel and buying a property here. Freshly baked diet-defying croissants, delicious cheeses, market fresh produce, and sprawling vineyards are all loveable clichés that attract millions of visitors to France each year.įrance’s relatively low property prices have more recently also become a major attraction for many visitors looking to continue their fond holiday memories abroad. Find a wide range of properties for sale in Dordogne, Nouvelle-Aquitaine, France, with FrenchEntre offering property for sale throughout France, find your. View online availability to chat everywhere you work - in email, a calendar event and on their contact card a. See your colleagues’ photos in your email. BlackBerry Work delivers a personalized business experience. You can also set up keyboard shortcuts for each profile to make it easier to swap between them if you so choose. This 5-minute guided tour of Photos Workbench rushes you past all the landmarks and leaves you wanting. 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Without a large local Chinese American community, the dim sum in Phoenix is fairly lackluster. » Read more: Our Ultimate Dim Sum Menu Guide with Pictures and Translations Today, Phoenix has neither a Chinatown nor any particular concentration of Chinese Americans, with the pace of new arrivals driven by the ebbs and flows of local professional employment opportunities. Following World War II, the community dispersed throughout the metropolitan area, all but abandoning the former downtown Chinatown. The community grew during the first half of the 20th century and opened a network of Chinese-owned groceries throughout the Phoenix area. Laborers working on the Southern Pacific Railroad established Phoenix’s first Chinatown during the late 1870s. Though Phoenix is yet to become a thriving regional hub of authentic Chinese cuisine, a small number of solid dim sum restaurants dot the city like welcome water stops in the heart of the Arizona desert. Whether it’s the sunshine or spring training that brings you Phoenix, take comfort that you’ll be able to satisfy your dim sum craving. If you’re a beginner, this section will bring you up to speed. If you already know the basics, skip this section. Let’s start with a basic overview and history of PostgreSQL and MySQL. The Basics: A General Overview of PostgreSQL and MySQL
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